WebJan 10, 2000 · Finally, cells were stained with (2′,7′-bis-(2-carboxyethyl)-5-(and-6-)-carboxyfluorescein (BCECF-AM, Molecular Probes, Leiden, The Netherlands) (fc 10 μM) for 30 min and after washing fluorescence activity was detected using the CytoFluor 2350 (Millipore, Bedford, MA) fluorescence measurement system . 2.5. Statistical analyses Webcells using a Cytofluor 2350 Fluore- s cent Plate Reader. The fluorescent units obtained from the microplate reader in-creased with increasing amounts of virus (Figure 2, left axis). Both the flow cytometric analysis and the relative fluorescent units from the fluorescent plate reader show an in-crease in EGFP-expressing cells with increasing MOI.
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WebJun 18, 2024 · The levels of DCF were measured using a CytoFluor 2350 fluorescence measurement system (Millipore) with an excitation wavelength at 485 nm (bandwidth 20 nm) and emission at 530 nm (bandwidth 25 nm). 6-Hydroxy-7- (3-hydroxypropyl)-4 ′ -methoxyisoflavone (1), C 19 H 18 O 5, obtained as an orange-yellow gum. WebNov 29, 2024 · Camptothecin (NSC-100880, CPT, Campathecin, (S)- (+)-Camptothecin) is a specific inhibitor of DNA topoisomerase I (Topo I) with IC50 of 0.68 μM in a cell-free assay. Camptothecin induces apoptosis in cancer cells via microRNA-125b-mediated mitochondrial pathways. Phase 2. Daunorubicin (RP 13057) HCl d3 mens volleyball statistics
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WebBCECF fluorescence was monitored with a CytoFluor 2350. The cytoplasmic pH was calculated from the ratio of fluorescence at excitation wavelengths of 485 and 440 nm. Dose-response curves were generated from single-cell intracellular pH measurements taken every 10 to 20 seconds for 20 minutes (data not shown). WebOct 3, 1995 · rescent product was measured using a Millipore Cytofluor 2350 fluorometer. AP42 ELISA A@,, was measured in a similarly formatted assay using 266 as the capture antibody. The reporter polyclonal antibody 277-2, was raised against a synthetic peptide that included AP residues 33 to 42 (GLMVGGVVIA), with cysteine- WebApr 1, 2001 · Fluorescence was quantified using the Cytofluor 2350 Fluorescence Measurement System (Millipore, Bedford, MA) at an excitation wavelength of 365 nm, a bandwidth of 40 nm, and an emission wavelength of 456 nm. Preparation of Plant Extracts and Protein Gel Blotting. g for 15 min. d3 minority\u0027s