Flp-intm t-rextm 293 细胞系

WebSep 29, 2024 · 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和MAPPIT (mammalian protein-protein interaction trap) 报告质粒获得，常用于蛋白互作筛选。 293S 细胞. 293S细胞支持悬浮培养，S为Suspension悬浮的首字母，可以耐受低Ca2⁺培养体系。 WebFigure 3-20. Relative expression of FLAG-hFFA4-mVenus and FLAG-hFFA4-GFP2 in a Flp-InTM T-RExTM 293 cell line. Flp-InTM T-RExTM 293 double stable cells able to express inducibly FLAG-hFFA4-mVenus and constitutively FLAG-hFFA4-GFP2 were seeded into poly-Dlysine coated black 96-well plates. Cells were treated with varying concentrations …

团队作案HEK293，史上最强搬砖细胞 - 简书

WebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows … WebC, immunoblots of cell lysates (input) used for FLAG immunoaffinity purification in B. D, MED21 lacking the first 5 amino acids is defective in TNFα-induced gene activation. Firefly luciferase activity was measured in lysates from Flp-InTM T-RExTM-293 cells stably expressing wild type or mutant MED21. c sharp output https://xtreme-watersport.com

Flp-In™ T-REx™ 293 Cell Line - Thermo Fisher Scientific

WebJul 2, 2012 · Als Kontrollen dienten mit dem Leervektor transfizierte und hCtr1 überexprimierende Flp-InTM T-RExTM-293-Zellen. Die rekombinanten hCtr2-Zellen erwiesen sich als signifikant weniger sensitiv gegenüber Cis- und Carboplatin als die Kontrollzellen. hCtr2 hatte hier also einen resistenzfördernden Effekt. Dies ist ein … WebHuman. The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. WebFlp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标（FRT）位点。随后，包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介导DNA重组，整合入基因组。 ead list c

Flp-In™ 系统 Thermo Fisher Scientific - CN

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Flp-intm t-rextm 293 细胞系

Flp Intmt Rextm 293 Cells Hek 293t Hek Human …

WebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × … Web丁香通为您提供FLPINTREX293商品详情介绍：价格：￥1 - 2000，货号：R78007，品牌：invitrogen,GIBCO，产地：美国，详见丁香通FLPINTREX293商品详情页；

Flp-intm t-rextm 293 细胞系

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WebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系，确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … Web293FTM细胞则是来源于Flp-InTMT-RETM293细胞，该细胞系中转染了ecotropic receptor质粒及MAPPIT(mammalian protein-protein interaction trap)报告质粒，该细胞系主要用于 …

WebAug 21, 2024 · 最近，有研究通过RMDI技术在Flp-InTM-293细胞中系成功地过表达核孔复合物亚基，以及在Flp-InTM-CHO细胞中表达P2Y12受体。 已有研究构建了具有两个不同重组位点（双重RMCE）的宿主细胞，用于在单个细胞系中过表达多个外源基因。 Web技术资料. 规格：. 1ml. Flp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。. Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶 …

WebFlp-InTM T-RExTM 293 cells stably harboring hFFA2-eYFP at the Flp-InTM T-RExTM locus were induced to express the receptor. The capacity of GLPG0974 to inhibit elevation of [Ca2+] i. produced by EC80 concentrations of C3 or Cmp 1 is shown (B). Membranes from such induced cells were used to assess the ability of GLPG0974 to inhibit the binding of ... WebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成 …

WebThermo Fisher flp in t rex human embryonic kidney 293 hek293t cell line Flp In T Rex Human Embryonic Kidney 293 Hek293t Cell Line, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... HEK293T-Flp-InTM T-RexTM (HEK293T) cells were maintained and cultured in DMEM media …

WebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... ead livro iWebT-REx™ 细胞系可稳定表达四环素抑制子蛋白（表 1）。使用 T-REx™ 系统可以节省大量的时间和精力。通过使用阳性对照载体 pcDNA™4⁄TO⁄lacZ 进行瞬时转染，对 T-REx™ 细胞系进行功能检测。在用四环素诱导时，T-REx™ 细胞系在抑制状态下表现出极低的基础表达水平，表达水平较高（图 1）。 ead login feobWebFigure 5-9. PMA induces internalisation of hFFA4. Flp-InTM T-RExTM 293 cells able to express FLAG-hFFA4-mVenus were plated down onto poly-D-lysine-coated 30 mm glass coverslips and receptor expression was induced using doxycycline (100 ng/ml). The internalisation of hFFA4 in live cells was then imaged using a Zeiss VivaTome spinning … ead liveclassWebgenomic location. To generate these cell lines, the Flp-In ™ T-REx System involves the following major steps: 1. Independent integration of the following two plasmids into the … csharp override methodead length of stayWebOct 31, 2024 · HEK293细胞及其衍生株HEK293（human embryonic kidney 293），简称293细胞，来源于人胚胎肾细胞，由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段，使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和 ... csharppadWebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成分EXOSC10（RRP6）的同基因HEK-293细胞系费雪科学）。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质，并且使用磁性 ... c sharp override gethashcode