Phospho-akt1/2/3 ser473 cellular kit
WebBath Filler and Heat Transfer Fluids; Bath Specific Platforms and Clamps; Bath Specific Tube Racks; Calibration Baths; Circulating Baths; Coliform Baths WebSep 12, 2015 · Inhibition CellularAkt semisyntheticviridins epidermalgrowth factor–dependent activation HT-29colon cancer cells Westernhybrid- ization using anti-phospho-Ser 473 -Akt antibody (Fig. 2A). Quantitation phospho-Ser473 totalAkt (Fig. 2B) gave IC 50 values (FSE) 2.2nmol/L, PX-867 3.5nmol/L,
Phospho-akt1/2/3 ser473 cellular kit
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Webphospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent Positive Control Cell Lysate (lyophilized) Detection Reagent (TMB Substrate) Stop Solution Plate seal Description … WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation
WebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ... WebThe Total SHP1 cellular assay monitors the expression level of SHP1, and can be used as a normalization assay with our phospho-SHP1 Y564 kit. Because these phospho and total SHP1 assays are compatible, the two kits can be used in parallel on the same lysates. Many cancer cells overexpress checkpoint inhibitor ligands such as PD-L1.
WebPhospho-ERK assay kits are designed for the robust quantification of ERK modulation, phosphorylated on Thr202/Tyr204, for GPCR and RTK targeted screening as a MAPK … WebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of …
WebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT …
Web(Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. Please visit www.cellsignal.com for a complete listing of recommended companion products. #7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit Species Cross-Reactivity: H, M, R … it is called as the venus of schelklingenWebAkt1/2 kinase inhibitor indeed decreased pAkt in all four cell lines , while total Akt levels were not affected by the treatment. To determine whether induction of apoptosis caused the decreased cell viability induced by the Akt1/2 kinase inhibitor, we measured the activity of caspase 3/7 in the treated cells by Caspase-Glo® assay. it is called the butt of arnis stickWebMyricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β nehru on cow slaughterWebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using … nehru nagar west coimbatoreWebMMP1 Antibody SKU: A... it is called as the shape slotted on a screwWebProduct Note This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey (>80% identity) PROPERTIES TARGET it is called law only figuratively speakingWebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. nehru nagar coimbatore which taluk