WebA targeting construct was designed to insert a stop codon, an IRES Tau-LacZ fusion protein and a loxP -flanked PGK-Neo cassette after the first valine (Val 33) in exon III, and also delete the remainder of exon III through exon IV of the targeted gene. This construct was electroporated into 129P2/OlaHsd-derived CGR8.8 embryonic stem (ES) cells. WebGeneration of EC2 tau-LacZ mouse line. A C57BL/6j mouse BAC array (RPCI-23, segment 2) was screened for EC2-containing bacterial artifi- cial chromosomes (BACs). Positive BACs were extensively analyzed by PCR, DNA sequencing, and restriction digests to assert the presence of the EC2 gene.
Development of P2 olfactory glomeruli in P2-internal ribosome …
WebJun 21, 1994 · We have constructed an axon-targeted beta-gal reporter by fusing the cDNA encoding the bovine microtubule-binding protein, tau, to lacZ, the E. coli gene encoding … WebApr 8, 2014 · Tau-lacZ codes for a protein composed of the beta-galactosidase enzyme fused to a signal sequence from tau (an actin-associated protein), which allows the … alfonza salamone
Visualization of functionally activated circuitry in the brain
WebFeb 8, 2002 · In mice heterozygous for tau-lacZ targeted to the melanopsin gene locus, beta-galactosidase-positive RGC axons projected to the SCN and other brain nuclei involved in circadian photoentrainment or the pupillary light reflex. Rat RGCs that exhibited intrinsic photosensitivity invariably expressed melanopsin. Hence, melanopsin is most likely the ... WebMar 25, 2009 · In tau-lacZ+/+ mice a subset of ipRGCs is identified by their expression of β-galactosidase (M1 ipRGCs) and these neurons send dendrites to the OFF substrata of the IPL where they stratify near the border of the inner nuclear layer (INL) [14]. WebSep 1, 1997 · UAS-tau-lacZ and UAS-ricin A Pioneer neurons were visualised by driving expression of the reporter gene, UAS-tau-lacZ ( Hidalgo et al., 1995 ). Targeted ablation was achieved by expression of UAS-ricin A ( Hidalgo et al., 1995; John, Davidson, Moffat, O’Kane, Halder, Gehring, Yoffe and A. H. B., unpublished data). misskey.io チャンネル